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Applications of BBa_K325905
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[http://2012.igem.org/Team:UC_Chile Team UC_Chile]
We tested the light emittion by this part depending on the addition of two aldehydes substrates: decanal and dodecanal. Our results show that E. coli transformed with this biobrick are unable to emit light when the decanal substrates are added, this can be due to an error in the promoter and/or in the LuxAB proteins.
Expression was measured on a Termo Scientific Luminoskan Ascent luminometer for microplates, for more details on the experimental design go to Luciferase Assay at our [http://2012.igem.org/Team:UC_Chile/Protocols protocols page].
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UNIQc5b283ddac7e8e8a-partinfo-00000000-QINU
UNIQc5b283ddac7e8e8a-partinfo-00000001-QINU
